Pattern formation in leaves via small RNA mobility

Pattern formation via small RNA mobility.

MicroRNAs and trans-acting siRNAs (ta-siRNAs) have important regulatory roles in development. Unlike other developmentally important regulatory molecules, small RNAs are not known to act as mobile signals during development. Here, we show that low-abundant, conserved ta-siRNAs, termed tasiR-ARFs, move intercellularly from their defined source of biogenesis on the upper (adaxial) side of leaves ...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

Analysis of Pattern Formation on the Surface of Respiring Leaves

The process of photosynthesis is facilitated in a plant by pores in its surface called stomata. When a particular stoma is open, CO2 is absorbed through its aperture, but H2O is also lost due to evaporation. Thus a plant will seek a stomatal aperture that balances its need for CO2 with its aversion to H2O loss. In order to visualize a particular leaf’s stomatal aperture distribution and how it ...

Small interfering RNA; principles, applications and challenges--

Gene silencing using RNAi (RNA interference), has recently been used as a successful laboratory technique in determining the function and control of gene expression and provides a wide range of applications in molecular biology and gene therapy. RNAi is a method of suppressing gene expression. In this direction, a single-stranded RNA molecule of about 21–23 nucleotides, called siRNA (small inte...

Controlling pattern formation in nanoparticle assemblies via directed solvent dewetting.

We have achieved highly localized control of pattern formation in two-dimensional nanoparticle assemblies by direct modification of solvent dewetting dynamics. A striking dependence of nanoparticle organization on the size of atomic force microscope-generated surface heterogeneities is observed and reproduced in numerical simulations. Nanoscale features induce a rupture of the solvent-nanoparti...